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    In addition to yielding abundant planting material of uniform age, micropropagated plants have been known to mature and fruit all at one time. Perhaps the use of micropropagated (tissue cultured) pineapple plants as planting material would help to minimize the problem of ‘natural flowering’ which is sporadic and rampant in pineapple . gzhwmzbsfc.ygto.com. improving the rate of plant multiplication and a faster multiplication of elite genotypes, led to the development of tissue culture techniques for the pineapple (Almedia, 1994). Bregonci et al. (2008) had as objective to evaluate the foliar and radicular growth of micropropagated plantlets of the pineapple cv. Gold [Ananas comosus (L.) Merrill]. Ms electronics engineering in pakistan politicannews. Three-stage protocol has been developed for rapid mass propagation of pineapple (Ananas comosus cv. Queen). In the first step cultures were initiated on MS-I media fortified with 0.1 mg/l NAA and . yxlfngsgvd.youdontcare.com. Globally, production of pineapple fruits reached more than 25 million tons in 2013. Therefore, many scientists are searching for new varieties and ways for the conservation of pineapple genetic resources. Cryoconservation in liquid nitrogen has been described as a suitable technology in many plant species. However, its potential effects in the subsequent plant growth in the field should be . Samsung tv hdd format exfat windows. Micropropagation is the practice of rapidly multiplying stock plant material to produce many progeny plants, using modern plant tissue culture methods.. Micropropagation is used to multiply plants such as those that have been genetically modified or bred through conventional plant breeding methods. It is also used to provide a sufficient number of plantlets for planting from a stock plant . Cirrus tablets indications. tion, infected pineapple plants showing a greater increase in shoot production in comparison to root production. micropropagated pineapple / arbuscular endomycorrhizal fungi / plant growth infection development Résumé — Sélection de champignons endomycorhizogènes à arbuscules efficients pour l’établissement d’ananas micropropagés. cybmgmhozq.changeip.co. These pineapple plantlets are easily rooted in hormone-free medium. The average survival of plantlets was over 95% under both environmental conditions. Our results and calculations (data not presented in the text) show that the cost-price of micropropagated plants under nethouse conditions are about 20% lower compared to growth room conditions. Browser preview ipad. of shoots and roots of micropropagated pineapple cv. ‘Vitória’ plants depended on inoculation with diazotrophic bacteria, and plantlets acclimatization could be reduced by up to 57 days, depending on the concentration of urea fertilizer. Key words: Ananas comosus, plant growth-promoting bacteria, nitrogen. RESUMO mmcsggwpti.mynetav.com. The objective of this work was to study leaf anatomy of pineapple plants in order to improve acclimatization protocols. In vitro plantlets weighting an average of 3.1 g and greenhouse plantlets . bihyigjhug.youdontcare.com. micropropagated grapevine rootstock and pineapple plants P Lovato, Jp Guillemin, S Gianinazzi To cite this version: P Lovato, Jp Guillemin, S Gianinazzi. Application of commercial arbuscular endomycorrhizal fun-gal inoculants to the establishment of micropropagated grapevine rootstock and pineapple plants. One picture multiple frames appleton. In the present work, pineapple plantlets (cv. `Amarelinho') micropropagated by stationary (S) and temporary immersion (T) systems were evaluated in terms of genotypic fidelity by isozyme and RAPD markers. Neither isozymes (average 0.67%) nor RAPDs (average 7.5%) alone detected significant differences between the two micropropagated systems. jjvzovhivy.freeddns.com.

    Micropropagation - Wikipedia

    Micropropagation is the practice of rapidly multiplying stock plant material to produce many progeny plants, using modern plant tissue culture methods.. Micropropagation is used to multiply plants such as those that have been genetically modified or bred through conventional plant breeding methods. It is also used to provide a sufficient number of plantlets for planting from a stock plant ... Table 2 - Plant height (PH), number of leaves per plant (NL), leaf area per plant (LA), shoot dry weight (SDW), and root dry weight (RDW) of micropropagated plantlets of the pineapple cv. 'Vitoria' that were either inoculated or not inoculated with diazotrophic bacteria. Average values of acclimatization time. The Food and Agriculture Organization has highlighted pineapple as one of the most important tropical fruits. Since classical pineapple breeding is difficult, biotechnology has emerged as an attractive instrument. We obtained two new pineapple somaclonal v...

    ACCLIMATIZATION OF MICROPROPAGATED PLANTLETS OF 'IMPERIAL ...

    The acclimatization period has been a critical period in this process, determining evolution of plant development and time needed for obtaining plantlets adequate for transfer to the field. This work was carried out to determine the effects of substrates and fertilizer doses on Imperial pineapple plantlets growth during acclimatization. Physiology of Effects of Temporary Immersion Bioreactors on Micropropagated Pineapple Plantlets Created Date: 20160802020628Z ...

    Screening of arbuscular endomycorrhizal fungi for ...

    tion, infected pineapple plants showing a greater increase in shoot production in comparison to root production. micropropagated pineapple / arbuscular endomycorrhizal fungi / plant growth infection development Résumé — Sélection de champignons endomycorhizogènes à arbuscules efficients pour l’établissement d’ananas micropropagés. The 'Imperial' pineapple cultivar stands out in the Brazilian scenario as an alternative for most of the production areas where the fusariosis disease causes high losses. The low availability of planting material has been one of the main limitations for pineapple growers and tissue culture has to be used intensively for meeting the demand of good quality planting material. ABSTRACT: Pineapple is one of the most important tropical crops worldwide. The use of temporary immersion bioreactors for the first stages of pineapple propagation enables precise control of plant growth, increases the rate of plant multiplication, decreases space, energy and labor requirements for pineapple plants in commercial micropropagation.

    MICROPROPAGATION AND GROWTH OF IN VITRO PINEAPPLE ANANAS ...

    improving the rate of plant multiplication and a faster multiplication of elite genotypes, led to the development of tissue culture techniques for the pineapple (Almedia, 1994). Bregonci et al. (2008) had as objective to evaluate the foliar and radicular growth of micropropagated plantlets of the pineapple cv. Gold [Ananas comosus (L.) Merrill]. Growth of Micropropagated Ananas comosus var. erectifolius Plantlets ... ornamental pineapple, vermiculite, fertilization, coconut mesocarp dust ... Micropropagation is a very helpful tool for plant propagation, as it provides uniform plants, with improved phytosanitary quality. These factors, as well as the use of Effects of in vivo mycorrhization on micropropagated fruit tree rootstocks Effects of in vivo mycorrhization on micropropagated fruit tree rootstocks Monticelli, S; Puppi, G; Damiano, C 2000-10-01 00:00:00 In order to establish an endomycorrhizal symbiosis on micropropagated plants and to evaluate its effect on plant growth and survival, three rootstocks belonging to the Prunus genus were used ...

    (PDF) Leaf anatomy of micropropagated pineapple plants

    The objective of this work was to study leaf anatomy of pineapple plants in order to improve acclimatization protocols. In vitro plantlets weighting an average of 3.1 g and greenhouse plantlets ... Micropropagation based techniques enable the mass production of pest- and disease-free stock plants. Aiming at optimizing pineapple [Ananas comosus (L.) Merril] micropropagation, a complete randomized experiment with three factors was established: immersion system (2 L twin flasks temporary immersion and permanent immersion); culture medium (supplemented or not with GA3); and shoot height ...

    PINEAPPLE (Ananas comosus) CV. PÉROLA ex vITRo GROWTH AND ...

    response of micropropagated pineapple plantlets to mycorrhizal inoculation varies with plant and fungal genotypes (SANTOS et al., 2011), meaning that specific procedures must be tested for each plant genotype. It has also been demonstrated that mycorrhizal inoculation can stimulate root development in micropropagated plants during the But such micropropagated plants ultimately come in association with either symbiotic or pathogenic organisms when they are planted in the field. If these plants are inoculated with appropriate symbiotic organisms prior to transfer to ex vitro condition, there is scope of improving plant survival and subsequent ex vitro growth (Sahay and Varma ...

    PHYSIOLOGY OF EFFECTS OF TEMPORARY IMMERSION BIOREACTORS ...

    Temporary immersion bioreactors are an efficient tool for plant mass propagation because they increase multiplication rate and plant quality. Little knowledge is available on the ecosystem and physiological behavior of plantlets when using this new culture technique. In order to evaluate the effects of the conditions on physiological change of pineapple plantlets, a factorial experiment was ... The Food and Agriculture Organization has highlighted pineapple as one of the most important tropical fruits. Since classical pineapple breeding is difficult, biotechnology has emerged as an attractive instrument. We obtained two new pineapple somaclonal variants derived from in vitro culture of cv. Red Spanish Pinar: P3R5 and Dwarf. The AFLP analysis revealed an existing genetic distance.

    Genotypic fidelity of micropropagated pineapple ... - DeepDyve

    Read "Genotypic fidelity of micropropagated pineapple (Ananas comosus) plantlets assessed by isozyme and RAPD markers, Plant Cell, Tissue and Organ Culture" on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. Project Methods To accomplish the task of pineapple micropropagation and commercial cultivation to enhance productivity in Micronesia successfully, the entire project plan, which comprises of five main objectives, is divided into three phases according to year wise time framing.In the first and second year of the project, the first three objectives will be taken up.

    SCREENING OF BIOACTIVE COMPOUNDS FROM BACTERIAL ENDOPHYTES ...

    The determination for bioactive compounds from bacterial endophytes was also carried out. Meterials and Methods Isolation of Bacterial Endophytes from Plant Samples (Kumrapich, 2012) Plant samples including rice and pineapple were obtained from plant tissue culture laboratory, Faculty of Science, Maejo University (Figure 1). Com o substrato contendo terra, esterco e Plantmax foi obtido o melhor desenvolvimento das raízes e parte aérea.
    The objective of this work was to evaluate the effect of the organic matter as component of substrata for micropropagated plants of pineapple cv. Pérola in the acclimatization phase.

    Genotypic fidelity of micropropagated pineapple ( Ananas ...

    In the present work, pineapple plantlets (cv. `Amarelinho') micropropagated by stationary (S) and temporary immersion (T) systems were evaluated in terms of genotypic fidelity by isozyme and RAPD markers. Neither isozymes (average 0.67%) nor RAPDs (average 7.5%) alone detected significant differences between the two micropropagated systems. Biological hardening. Kavino et al. Table 1 Beneficial microorganisms used as inoculants in various plantlets and its significance on plant characters. Bio control agents Crop (micropropagated) Method of inoculation Significance Reference Bacillus sp. and Pseudomonas corrugata Tea Ex vitro Improving the survival rate of seedlings Pandey et al. 2000 ...

    Application of commercial arbuscular endomycorrhizal ...

    micropropagated grapevine rootstock and pineapple plants P Lovato, Jp Guillemin, S Gianinazzi To cite this version: P Lovato, Jp Guillemin, S Gianinazzi. Application of commercial arbuscular endomycorrhizal fun-gal inoculants to the establishment of micropropagated grapevine rootstock and pineapple plants. (1) are endophytic bacteria present in micropropagated pineapple plants, maintained in vitro for long periods of cultivation? (2) are these bacteria equally distributed throughout the plant organs? Materials and methods Samples were collected from pineapple microplants (Ananas comosus (L.) Merrill, cv. IAC Gomo-de-mel) from

    Field performance of micropropagated and cryopreserved ...

    Globally, production of pineapple fruits reached more than 25 million tons in 2013. Therefore, many scientists are searching for new varieties and ways for the conservation of pineapple genetic resources. Cryoconservation in liquid nitrogen has been described as a suitable technology in many plant species. However, its potential effects in the subsequent plant growth in the field should be ... In the present work, pineapple plantlets (cv. `Amarelinho') micropropagated by stationary (S) and temporary immersion (T) systems were evaluated in terms of genotypic fidelity by isozyme and RAPD markers. Neither isozymes (average 0.67%) nor RAPDs (average 7.5%) alone detected significant differences between the two micropropagated systems. Globally, production of pineapple fruits reached more than 25 million tons in 2013. Therefore, many scientists are searching for new varieties and ways for the conservation of pineapple genetic resources. Cryoconservation in liquid nitrogen has been described as a suitable technology in many plant species.

    Diazotrophic bacteria and nitrogen fertilization on the ...

    of shoots and roots of micropropagated pineapple cv. ‘Vitória’ plants depended on inoculation with diazotrophic bacteria, and plantlets acclimatization could be reduced by up to 57 days, depending on the concentration of urea fertilizer. Key words: Ananas comosus, plant growth-promoting bacteria, nitrogen. RESUMO Endomycorrhization of pineapple vitroplants at outplanting from in vitro conditions positively affected their mineral nutrition (biofertilizer), growth and contents of photosynthetic pigments; the fungus Glomus sp. (LPA21) was the most effective. A negative correlation was observed between shoot stimulation and root development indicating a ... signal molecule as plant response against many abiotic and biotic stress (Schilmiller and Howe, 2005; Abdala and Cenzano, 2006), could attenuate these effects in pineapple plantlets during in vitro-ex vitro transition and SOD activity could be a biological indicator, whose demonstration is the objective of this work. The

    High Light Intensity Increases the CAM Expression in “MD-2 ...

    Pineapple (Ananascomosus (L.) Merr.) is a perennial monocot plant belonging to the family Bromeliaceae, sub-family Bromelioideae, in the order Bromeliales. Pineapple is one of the most economically important tropical fruits [1] and the most of CAM plants [2]. After bananas and mangoes, it is the third most important tropical characterize the development of two ornamental pineapple hybrids as potted plant. Two accessions (‘A’ and ‘D’) of ornamental pineapple from the Pineapple Germplasm Bank of Embrapa Cassava & Tropical Fruits were used. The acclimatization of micropropagated plants lasted 60 days and afterwards plantlets

    ENDOMYCORRHIZA BIOTECHNOLOGY AND MICROPROPAGATED PINEAPPLE ...

    Micropropagation produces plants without disease but also suppresses endomycorrhiza formation. Experiments were carried out with the ‘Smooth Cayenne’ variety of pineapple and with five endomycorrhizal fungi under a simulated tropical environment. American Journal of Plant Sciences 2015 . High Light Intensity Increases the CAM Expression in “MD-2” Micro-Propagated Pineapple Plants at the End of the Acclimatization Stage. DOI: 10.4236/ajps.2015.619303, PP. 3109-3118.

    High Light Intensity Increases the CAM Expression in “MD-2 ...

    This work describes the evaluation of morpho-physiological and biochemical changes in “MD-2” micro-propagated pineapple plants (Ananas comosus (L.) Merr.) grown after 30 days under low light intensity (LL, greenhouse light conditions at 250 μmol·m-2·s-1) or high light intensity (HL, field light conditions at 800 μmol·m-2·s-1). Gas exchange, leaf pH, protein content and superoxide ... Abstract. Several arbuscular endomycorrhizal fungi (Glomus clarum (LPA16), Scutellospora pellucida (LPA20), Glomus sp. (LPA21), Glomus sp (LPA22) and Glomus sp (LPA25)) were tested for plant growth effects and infection development in Queen Tahiti, Smooth Cayenne (clone CYo) and Spanish varieties of micropropagated pineapple growing in an acid soil under growth chamber tropical conditions.

    Studies on Micropropagation of Pineapple (Ananas comosus L.)

    Three-stage protocol has been developed for rapid mass propagation of pineapple (Ananas comosus cv. Queen). In the first step cultures were initiated on MS-I media fortified with 0.1 mg/l NAA and ... Acclimatization of micropropagated plants to ex vitro conditions J. POSPÍŠILOVÁ*, I. TICHÁ**, P. KADLEČEK**, D. HAISEL* and Š. PLZÁKOVÁ** Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Na Karlovce 1a, CZ-16000 Praha 6, Czech Republic* Charles University, Faculty of Science, Department of Plant Physiology, A number of researchers have reported plant regeneration via organogenesis and embryogenesis in pineapple (Table 2). Pineapple was first micropropagated in vitro by Aghion and Beauchesne (1960). Shoot apices of pineapple have been cultured using different growth regulators at various Table 2 Studies on in vitro regeneration in pineapple.

    Micropropagation of pineapple, Ananas comosus (L.) Merr ...

    In addition to yielding abundant planting material of uniform age, micropropagated plants have been known to mature and fruit all at one time. Perhaps the use of micropropagated (tissue cultured) pineapple plants as planting material would help to minimize the problem of ‘natural flowering’ which is sporadic and rampant in pineapple ... Pineapple (Ananascomosus (L.) Merr.) is a perennial monocot plant belonging to the family Bromeliaceae, subfamily Bromelioideae, in the order Bromeliales. Pineapple is one of the most economically important tropical fruits [1] and the most of CAM plants [2] . hardening of micropropagated plants and finally growing them in the field. This publication describes options for reducing costs to establish and operate tissue culture facilities and primarily focus on plant micropropagation. It includes papers on the basics of

    The interaction between arbuscular mycorrhizal fungi and ...

    Content (mg plant −1) of P, N, K, Mg and Ca in micropropagated pineapple plantlets inoculated with C. etunicatum, D. heterogama, R. clarus, P. indica, Mix or control. Measurements were obtained 210 days after inoculation in the greenhouse with different P levels in the soil. °, * and **, significant at 10, 5 and 1% probability, respectively. Sanford, W.G. (1961) Key to the visual nutrient deficiencies on pineapple plants with colour and black nd white photographs. Research Report no. 85, Pineapple Research Institute of Hawaii, Honolulu. Sanford, W.G. (1962) Pineapple crop log-concept and development. Better Crops With Plant Food 46, 32-43. Scott, C. (1993) The effects of: 1.

    Potential low-cost micropropagation of pineapple (Ananas ...

    These pineapple plantlets are easily rooted in hormone-free medium. The average survival of plantlets was over 95% under both environmental conditions. Our results and calculations (data not presented in the text) show that the cost-price of micropropagated plants under nethouse conditions are about 20% lower compared to growth room conditions. In Micronesia, non-availability of disease-free and elite seedlings, shortage of trained agricultural professionals, and limited skills of farmers for commercial production have resulted in limited pineapple production.Therefore, this project is specifically designed to optimize micropropagation and nursery management systems to produce elite seedlings in bulk quantities, and provide ... Acclimatization of micropropagated seedlings of pineapple cultivars on organic substrates - Read online for free. Ananas comosus var. comosus; abacaxizeiros ‘Vitória’, ‘Pérola’ e ‘Imperial’; cultura de tecidos de plantas; micropropagação; multiplicação de plantas. Materia orgânica; substratos orgânicos;